Correction: Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response
نویسندگان
چکیده
Reference 1. Theilacker C, Diederich A-K, Otto A, Sava IG, Wobser D, Bao Y, et al. (2015) Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response. PLoS ONE 10(7): e0132949. doi: 10.1371/journal.pone.0132949 PMID: 26172831 Fig 3. Stimulation of TNF-α production in RAW 264.7 mousemacrophages by lipoprotein-enriched cell membrane fractions of E. faecaliswild type and ΔbgsA.RAW264.7 cells were incubated with lipoproteinenriched Triton X-114 extracts from total membrane protein fractions derived from the indicated E. faecalis strains. The concentration of lipoprotein extracts was measured photometrically and normalized to a bacterial cfu:RAW 264.7 cell ratio of 10,000:1. At 16 h, supernatants were collected and TNF-α concentrations were quantified by ELISA. LPS at a concentration of 100 ng/ml was used as positive control. Data represent mean ± SEM of triplicates. * p < 0.001 12030 ΔbgsA versus 12030WT.
منابع مشابه
Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response
In this study, we investigated the impact of the cell membrane composition of E. faecalis on its recognition by the host immune system. To this end, we employed an E. faecalis deletion mutant (ΔbgsA) that does not synthesize the major cell membrane glycolipid diglycosyl-diacylglycerol (DGlcDAG). Proteomic analysis revealed that 13 of a total of 21 upregulated surface-associated proteins of E. f...
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عنوان ژورنال:
دوره 10 شماره
صفحات -
تاریخ انتشار 2015